Direct from Positive Blood Culture

Sample preparation for the LifeScale-AST for samples direct from positive blood culture should take less than 10 minutes and is achieved using standard broth microdilution techniques and the LifeScale-AST kit.

Take a 1.5ml aliquot from the positive blood culture bottle and confirm Gram-negative Gram-Stain
Add the 1.5ml aliquot to the 1.5 ml vial of growth media and centrifuge for 5 minutes at 1000g
Take 1 ml of supernate and add it to the 12.5ml vial of growth media from the LifeScale-AST kit. The LifeScale-AST will determine the bacterial load, dilute the inoculum to 4.5x105, and inoculate the well plate.

Place the film cover on the plate and place the plate on the LifeScale-AST instrument