From Isolate

Sample preparation for the LifeScale-AST for samples direct from positive blood culture should take less than 5 minutes and is achieved using standard broth microdilution techniques and the LifeScale-AST kit.

Touch 3-5 colonies from a blood agar plate and suspend in 5 ml of sterile saline
Vortex the sample for 30 sec.
Take 1 ml of inoculum and add it to the 12.5ml vial of growth media from the LifeScale-AST kit. The LifeScale-AST will determine the bacterial load, dilute the inoculum to 4.5x105, and inoculate the well plate.

Place the film cover on the plate and place the plate on the LifeScale-AST instrument